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youth and adults has declined significantly (112-113)
Samples were then centrifuged for 5min at 13,000rpm and the supernatant was removed
CRH-R2, as noted above, binds the urocortins 1, 2 and 3, and appears to mediate a down-regulatory role in the HPA response to stress: knockout mice exhibit a hypersensitive acute ACTH and corticosterone response (198) and a defective recovery from stress with a slower drop in corticosterone (199)
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