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Your body can use them immediately

Blood samples for analysis of serum B12 should be protected from light during collection, and whole blood separated as soon as possible as delays in centrifugation and separation can lead to an initial rise in serum B12 concentrations (Allen et al, 2018)

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Vitamin B12 deficiency often leads to nervous system damage that damages the optic nerve leading to your eyes

However, if you have a large family, poor water quality, or notice changes in taste, odor, or water flow, you may need to replace it more frequently sometimes every 3-4 months