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Lambs: 0.5 ml
Others wait until the patient reports either reduced efficacy or increased side effects
However, individual responses vary
Protein concentration of the cell extracts was determined by Bradford assay (Bio-Rad Laboratories, CA, USA)
"Pharmaceutical companies will say there are drugs which appear to have potential but never go on beyond an early development phase, so this means the whole process of interpreting data and how things can be categorised under the WADA list is difficult and subject to interpretation." - with Jon Ralph
If you exercise or live in a hot climate, increase accordingly
