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Screening and live imaging of embryos and larvae were performed after anesthesia using 0.02% tricaine (Sigma-Aldrich)

Mechanistically, Tagitinin C induces oxidative stress and endoplasmic reticulum (ER) stress, leading to Nrf2 nuclear translocation and subsequent expression of its downstream target, HO-1

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Repeat this process until your buffer is the desired pH

Multivariate ANOVA for repeated measures (RM MANOVA) revealed a significant test time group interaction (P , 0.05) for changes in CORT, IL-6 and 8-iso from trial 1 to trial 2

polyphenol metabolites may be excreted in bile and undergo enterohepatic cycling, prolonging their presence [113]