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A further methodological issue is that many in vivo studies explicitly exclude female animals to avoid potential confounding effects related to hormonal fluctuations

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doi:10.1254/jphs.11r04fm

The long chain fatty acids first bind to CoA to form the fatty acyl CoA that in turn binds with L-carnitine, and the resulting acylcarnitine is shuttled through the inner membrane and into the mitochondria (Figure 2)

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