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On the day of the assay, serial dilutions (7-fold dilutions, 7 concentrations per compound and one well containing only assay buffer) of test compounds were performed in duplicate in assay buffer to appropriate concentrations in 96-well plates (Greiner, U-shape #GR-650201) using a Biomek i7 liquid handler

So, it is important to do check-ups and treat a deficiency as soon as possible

This research received funding from the clinical medical research center for obstetrics and gynecology diseases in Qinghai Province (Grant No

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For the reconstituted solution, it causes structural degradation that no visual inspection will catch