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doi: 10.3390/life13102061 30

A ketogenic diet improves mitochondrial biogenesis and bioenergetics via the PGC1alpha-SIRT3-UCP2 Axis

two studies assessed this parameter [26, 28]

Effects of Transcutaneous Vagus Nerve Stimulation in Individuals Aged 55 Years or Above: Potential Benefits of Daily Stimulation. Aging (Albany NY) 11: 48364857

Four m-thick longitudinally-oriented sections including metaphysis were deparaffinized and rehydrated, and then processed for hematoxylin and eosin (H&E) (Thermo Scientific), tartrate resistant acid phosphatase (TRAP) staining (Sigma-Aldrich) and immunohistochemical staining using primary antibodies against Osterix (Abcam, 1:200 dilution) or Osteocalcin (Takara, 1:200 dilution)