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Equal amounts of the detergent-soluble lysates were resolved using the NuPAGE Novex Midi Gel system on 412% BisTris gels (Invitrogen), transferred to PVDF membranes (PerkinElmer) in between six pieces of Whatman paper (Fisher Scientific) set in transfer buffer from Biorad with 20% methanol, and following transfer and blocking in 5% nonfat milk in PBS, probed overnight with the antibodies listed above

Autologous nerve grafts have been considered as the best standard options for repair [3]

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Vescovo used the Culture novels as source material when naming the ship, sub, ROVs and other support vehicles

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