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10.4103/0973-029X.92965 86 PurohitB

Smoking has a complex relationship with thyroid autoimmunity it appears to mildly protect against Hashimotos while increasing risk of Graves disease

Briefly, the mCherry gene on pET28a was expressed in Escherichia coli BL21, and purified using the His-Trap HP column (GE Healthcare, USA) and eluted with elution buffer B (20 mM Tris-HCl [pH 7.5], 500 mM NaCl, and 500 mM imidazole)

doi: 10.1002/ijc.11644