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Moreover, vitamin C increases synthesis and secretion of melanin 175 and by the improvement of antioxidant defense capacity of melanocytes significantly contributes to melanogenesis prevention 46

Treatment with TNF did not affect iron availability, whereas it did enhance the expression of several regulators associated with GSH biosynthesis, including solute carrier family 7 member 11 (SLC7A11), a functional subunit of system Xc- and a molecule responsible for the generation of intracellular GSH, GCLC (a glutamate-cysteine ligase regulatory subunit) and GCLM (a glutamate-cysteine ligase catalytic subunit) (81)

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The samples for the proteome analysis were instead dried down and stored at 80C until subjected to nLC-MS analysis

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