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According to Wirdefeldt et al

Ecotoxicology 22:584596 Hasanuzzaman M, Hossain MA, Fujita M (2010) Physiological and biochemical mechanisms of nitric oxide induced abiotic stress tolerance in plants

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Avidin beads (avidin-agarose from egg white, 1.1 mg ml 1 in aqeuous glycerol suspension, Sigma-Aldrich) were carefully resuspended by inversion on an Eppendorf tube wheel at 4 C and transferred to an Eppendorf tube