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For analysing DNA/RNA content, cells were collected and stained for surface markers before being fixed with 4% paraformaldehyde for 30 min at 4 C, followed by a permeabilization step with FoxP3 permeabilization solution (eBioscience)

When to stay at a dose longer The standard schedule says to increase every 4 weeks

Xiao Z, La Fontaine S, Bush AI, Wedd AG (2019) Molecular mechanisms of glutaredoxin enzymes: versatile hubs for thiol-disulfide exchange between protein thiols and glutathione

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40 (13), 000999-20e120