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Collagen synthesis in human skin fibroblasts is stimulated by a stable form of ascorbate, 2-O-alpha-d-glucopyranosyl-l-ascorbic acid

They were washed once with cold ddH 2 O and resuspend with HEPES buffer, after 20 min the yeast was spun down, and the supernatant was measured with filters 485ex 520em on a plate reader (DTX880, Multimode Detector, Beckman Coulter)

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16 Clomiphene citrate can be effective in women with normal estrogen levels and normal or suppressed FSH levels during the follicular phase (first half) of the menstrual cycle, women with luteal phase deficiency, PCOS, and unexplained infertility

In contrast, two genes and their proteins, AGPAT5 (1-Acylglycerol-3-Phosphate O-Acyltransferase 5) and AOX1 (Aldehyde Oxidase 1), were downregulated