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[DOI] [PMC free article] [PubMed] [Google Scholar] 171.Chiarini A., Armato U., Gardenal E., Gui L., Dal Pr I

mTORC1 Activator SLC38A9 is required to efflux essential amino acids from lysosomes and use protein as a nutrient

Although, more recently, the silicon model study of the in vivo nutrient microenvironment of degenerative intervertebral disc has been found nutrient concentrations may increase by a reduction in diffusional distance due to reduced disc height and vasculature ingrowth 57

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FAM-Peptide and protein stock solutions were diluted to the desired concentration with assay buffer (10 mM HEPES, pH 7.4, 1 mM CaCl 2 , 150 mM NaCl, 0.1% Pluronic)