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It also independently stimulates growth factors through the wound-healing response

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In chronic toxicology studies in rats (6 months) and cynomolgus monkeys (9 months), the potential toxicity of AOD9604 was assessed after daily oral gavage

Western blotting Cells were washed once with cold PBS and lysed in RIPA buffer (50 mM Tris-HCl pH 7.5, 125 mM NaCl, 1% NP-40, 0.25% Na-deoxycholate, 1mM Na-fluoride, 1 mM Na orthovanadate, phenylmethanesulfonylfluoride [PMSF], and 1% protease inhibitor cocktail), vortexed, and incubated on ice 20

In rodent models, BPC-157 accelerates healing of transected tendons and injured skeletal muscle, consistent with enhanced fibroblast/tendocyte activity and collagen remodeling