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This value exceeds previously reported values of 2.3, 5.2, and 3.0%, pointing out potential regional changes in M

Fatty acid elongase 6 is an endoplasmic reticulum enzyme that inhibits GPX4 and GSH expression and ferritin heavy chain 1 (FTH1) activity by interacting with ACSL4, leading to ROS accumulation [142, 143]

doi: 10.1016/j.jfca.2023.105865

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Given its critical role in BBB function and disease pathophysiology, GLUT1 remains a promising target for CNS drug delivery and therapeutic intervention [62]

After that, the mixture was transferred into the column and pre-washed with wash buffer (PBS, contained 0, 10, or 30 mmol/L imidazole, pH = 7.6), followed by elution buffer (PBS, contained 300 mmol/L imidazole, pH = 7.6)