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The longest-duration study we've found in peer-reviewed literature followed participants for 84 days

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B12 is required for the synthesis of red blood cells, growth and a childs development, maintaining the nervous system and synthesis of DNA during cell division, which is especially important in regions of rapid cell division such as bone marrow (red blood cell formation)

However, for those supplementing, high B12 levels are generally a reflection of recent supplementation, not a cause for concern

Vitamin B12, folate, and homocysteine in depression: the Rotterdam Study