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A fragment of the pcDNA3-LAP-Renilla between the BglII restriction site and spanning the renilla sequences was amplified by PCR using the following primers, forward : 5-CGG AAA TGT TGA ATA CTC ATA CTC-3 and reverse : 5-GCT CAG ATC TCC TCA GAA GCC ATA GAG C-3

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Gut Microbiota Regulates Bile Acid Metabolism by Reducing the Levels of TauroBetaMuricholic Acid, a Naturally Occurring FXR Antagonist. Cell Metabolism 17: 225235

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Except from ramoplanin and compound 5b, the binding of the proteins or the compounds have no significant effect on the fluorescence intensities (within the experimental error)