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The basic formula is straightforward: Final Concentration = Total Peptide Amount / Total Diluent Volume Lets use a common scenario

Measuring thiopurine metabolites in myeloid cells proved to be challenging however it was discovered that thiopurines metabolites are absorbed into red blood cells (RBC) and therefore metabolite measurement using RBC levels provided a practical surrogate for myeloid metabolite levels [55]

doi: 10.1038/sj.bjp.0706507

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10.1016/S0008-6363(01)00399-6 93 MorrisonD

In contrast, LDH-B transform lactate into pyruvate, allowing cells to use lactate as a source of nutrients for oxidative metabolism (26)