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Membranes were blocked in 5% non-fat milk for 1 h and then incubated with acetyl histone H4 (Upstate Biotechnology Corp., Waltham, MA, USA), dephosphorylated -catenin (Alexis, San Diego, CA, USA), phosphorylated p65 (Pharmingen, San Diego, CA, USA), p21 (Santa Cruz, CA, USA), survivin (Santa Cruz), cyclin D1 (Santa Cruz), PARP p89 (Santa Cruz), Bcl-XL (Santa Cruz), NF-B (Santa Cruz), and -actin (Santa Cruz) primary antibodies at 4C overnight

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Symptom scores were stable in both groups during this phase, indicating 45 days of a very-low-carbohydrate ketogenic diet followed by 45 days of a low-glycemic and insulinemic diet had a lasting benefit that could not be achieved by 90 days on a low-glycemic and insulinemic diet

Moreover, it delays muscle fatigue and boosts endurance