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(2) Redox recycling, in which GPx oxidizes GSH to glutathione disulfide (GSSG), followed by NADPH-dependent reduction back to GSH via glutathione reductase

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After incubation for 4 h at 37 C, the steroids were extracted with chloroform, evaporated by centrifugal concentrator, and re-dissolved in methanol for liquid chromatographymass spectrometry (LCMS) analysis, using buffer A containing 1 mM ammonium formate and buffer B containing 100% methanol

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[DOI] [PubMed] [Google Scholar] Susanto, H