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the GHK-Cu copper-binding tripeptide reference compound is synthesized with a confirmed 1:1 copper-to-peptide ratio verified by mass spectrometry, which is an important variable in receptor-binding and gene-modulation studies

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Surgical procedures Mice underwent a bilateral adrenalectomy (ADx) under anesthesia with isoflurane inhalation

Furthermore, GSH cannot cross cell membranes directly

Conflict of interest The authors report no conflict of interest

Consistently, FIDAS-5 treatment markedly reduced lactate production in both MNNG/HOS and U-2OS cells (Fig