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LPL is first synthesized as a proenzyme in the rough endoplasmic reticulum (ER), where it is later glycosylated and folded in the presence of calnexin (CXN) and lipase maturation factor 1 (LMF1) ( Lipoprotein Lipase Inhibition by Angiopoietin-Like 4 Once tethered to the cell surface or capillary lumen the activity of LPL is also dependent on the expression and availability of endogenous activators and inhibitors

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