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The main limitations are GI side effects: nausea, constipation, and slowed gastric emptying
doi: 10.1006/bbrc.2001.5977
www.nature.com, Lee, Su-Jun, and Joyce A
A representative example of gating strategy used to characterize FASN lox/lox -PyMT MEFs in apoptosis in 2D cultures, by using co-staining with Annexin-V and DAPI is shown in Supplementary Fig
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