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37 However, most solid tumor cells exhibit markedly reduced CAT expression due to chronic oxidative stress, high iron burden, and specific genetic mutations, accompanied by impaired glutathione (GSH) antioxidant systems

Documented pre-existing humoral and cellular immunity to AAV capsids and frequently utilized Cas orthologs (SpCas9, SaCas9) can neutralize vectors or induce T-cell-mediated clearance of edited cells

Moreover, following the injection of pancreatic cancer cells with knockout of OAT and ODC1 (encoding ODC protein) into immunodeficient mice, the resulting tumor volume showed no significant difference compared to that in control mice