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Taking cues from these approaches, our group designed a biotin linker consisting of three parts: 1) a biotin moiety, 2) a Boc-derivative cleavable by TFA, and 3) an alkyne group for bioorthogonal ligation with azide-functionalized sulfenic acid probes

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MMP, a key indicator of mitochondrial functionality, was assessed using JC-1 dye, which differentiates between energized and depolarized mitochondria
