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Additionally, the procedures reversibility and adjustability allow for progressive treatments tailored to individual goals and comfort levels

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Before the experiments, the samples were thawed at 4C until no ice was observed in the tubes

Density increases from chylomicrons (CM, of lowest density) through lipoproteins of very low density (VLDL) , intermediate density (IDL) and low density (LDL), to high density lipoproteins (HDL)

Samples were then transferred to 0.2 m centrifugal filters and centrifuged again at 10,000 RCF for 5 min at 4C before being transferred to glass auto-sampler vials ready for injection