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After 12 h of incubation, a working concentration of EDU reagent was added and incubated for another 2.5 h

Keywords Liver disease, Gutliver axis, Gut microbial dysbiosis, Microbial metabolites, Gut barrier, Ecological niche remodeling, Therapeutics, Multi-omics Introduction Liver diseases are one of the leading causes of death and disability worldwide, with a broad spectrum ranging from metabolic dysfunctionassociated steatotic liver disease (MASLD)/nonalcoholic fatty liver disease (NAFLD), alcoholic liver disease (ALD), and viral hepatitis to cirrhosis and hepatocellular carcinoma (HCC)

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Griffiths SK, Campbell JP (2015) Placental structure, function and drug transfer