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histologically, they appear as distended, weakly-staining cells with large, realtively clear nuclei) and brain edema were described and evaluated semiquantitatively (22)
[DOI] [PubMed] [Google Scholar] 38.Hernando S., Herran E., Figueiro-Silva J., Pedraz J.L., Igartua M., Carro E., Hernandez R.M
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For activity measurements, neurons (6-8 weeks in culture) were stimulated with 200 action potentials, 40 Hz 5 s using a field stimulation chamber RC-47FSLP (Warner Instruments) and imaged at physiological temperature (37 C) in an imaging buffer 88 containing 1.3 mM Ca 2+ with an epifluorescence microscope Nikon Eclipse Ti equipped with a 40X oil objective
[62] PHA-665752 (Pfizer) specifically inhibits MET kinase activity, and it has been demonstrated to represses both HGF-dependent and constitutive MET phosphorylation

But check for any cracks in the glass, particularly hairline fractures that might not be immediately visible