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Injecting Trimix too quickly may increase the risk of pain or bruising at the injection site

Once opened, refrigerate if you want to extend shelf life, though unopened bacteriostatic water is stable at room temperature

Sold through unregulated channels, it lacks safety data, quality control, and proven efficacy

Another limiting factor includes the implementation of dietary therapy, which many families find very tedious to implement, so families should be counseled before implementing dietary therapy in order to obtain desired results [96, 171] (Table 2 )

Overall, although limited, the evidence suggests a benefit of methyl-B12 for children with ASD