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Alcohol and Acetaldehyde Analysis in Serum Alcohol and acetaldehyde concentrations were measured in accordance with the Guidelines for Human Clinical Trials of Hangover Cure Claims established by the Ministry of Food and Drug Safety in Korea [22]

Dual luciferase assays were performed for relative luciferase activity in cell groups co-transfected with the pmiRGLO plasmids containing wild type (wt) or mutant type (mt) of MALAT1 (MALAT1-wt, MALAT1-mt), siRNA targeting MALAT1(si-MALAT1) miR-155-5p inhibitor or their control was transfected into KGN cells using Lipofectamine 2000

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These systems also allow for sustained release, maintaining steady peptide concentrations in the bloodstream and enhancing efficacy [68]
