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Protein digestion Protein concentration was estimated by BCA assay (Pierce) and the lysates were digested with Lys-C (Wako) in an enzyme/protein ratio of 1:100 (w/w) for 1 h followed by a threefold dilution with 25 mM Tris, pH 8.5 to 2 M GdmCl and further digested overnight with trypsin (Sigma-Aldrich) 1:100 (w:w)

Kawas et al

People who are deficient often notice a meaningful difference over the following days as levels recover, while those whose B12 is already fine may feel little change

The contributuion of amphetamine metabolites of (-)-deprenyls to its antiparkinsonian properties

2009;4(2) doi: 10.1371/journal.pone.0004449.e4449 [DOI] [PMC free article] [PubMed] [Google Scholar] 128.Hariharan S, Gustafson D, Holden S, et al
