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Furthermore, myosin light chain kinase (MLCK) acts with peri-junctional actomyosin rings to regulate the contractility of actin fibers, thereby influencing TJ structure and permeability (7, 8)

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a combination of 1mM IBMX, 1 M dexamethasone, and 10 g/ml of insulin in media were added to fully confluent 3T3-L1 fibroblasts for three days (days 03) to initiate differentiation

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