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Then cells were washed with cacodylate buffer 0.1 M and then post-fixed with 1% osmium tetroxide in water for 1 h at RT

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25 units per day on a 1 ml insulin syringe, equal to 1 mg per day at the standard reconstitution (10 mg vial in 2.5 ml)

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It exhibits potent antioxidant properties by enhancing endogenous defense systems, thereby mitigating oxidative neuronal damage a central event in AD pathogenesis (Heo et al