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For both targets, the assay was evaluated over a concentration range of 0.313 to 320 M and was demonstrated to have a quantitative dynamic range spanning nearly three orders of magnitude, with lower limits of quantification being 0.330 M for GSH and 0.370 M for cysteine

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This functional convergence demonstrates that ChaC1 activation, either achieved through genetic manipulation or pharmacological induction, creates a synthetic lethal context for AUR in HCC cells

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Among several Pichia and Wickerhamomyces isolates, Pichia kudriavzevii RWT exhibited the strongest tolerance, maintaining growth at AFB1 concentrations up to 200 ppb and decontaminating 26.5% of AFB1 within six hours, more than twice the reduction achieved by the next best isolate

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