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At 72 hours, cells were imaged under brightfield microscopy, and cells were lysed with Trizol Reagent (Thermo Fisher Scientific, Halethorpe, MD)
These metabolic adaptations during fasting, that are triggered by PPAR, serve to minimize the use of protein and carbohydrates as fuel to allow survival during long periods of energy deprivation
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Archived from the original on 13 December 2020
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