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A possible explanation for unexpected or contrasting observations is that forcing the plant to overproduce PCs caused a severe GSH depletion, which disables GSH in fulfilling its other important functions in antioxidative defense and signaling

Finally, the filtered cell lysate was centrifuged at 4 C for 10 min at 10,000 g

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Step 3: Moisturize Follow with a light moisturizer to lock in hydration