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However, many researchers still prefer "local administration" variables in their testing protocols to ensure maximum concentration at the specific tissue site
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A 0.8-cm defect in a lean, low-risk patient differs from a 2.5-cm defect in a patient with obesity, diabetes, chronic cough, and rectus diastasis

Immunostaining To perform the immunofluorescence staining against myelin antigens, the sections were washed three times with PBS containing 0.05% Tween 20, then incubated in blocking solution for 1 h at room temperature

doi: 10.1152/ajpcell.00558.2003

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