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The best effects were seen in trials that included women experiencing five or more hot flashes per day, those that used 80 mg or more of red clover isoflavones per day, those reporting higher concentrations of the isoflavone biochanin A, and those trials lasting 12 weeks or longer

An antigenic atlas of HIV-1 escape from broadly neutralizing antibodies distinguishes functional and structural epitopes

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doi: 10.1159/000321508

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The supernatant was then analyzed by HPLC with a Rainin Econosphere ODS C18 column and an acetonitrile/water mobile phase containing 0.1% trifluoroacetic acid