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Rapid acidification is required to prevent GSH auto-oxidation, GSH and GSSG degradation, as well as to precipitate proteins that interfere with analysis

10.1007/s11481-009-9171-5 152 HensleyK.FedynyshynJ.FerrellS.FloydR
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Precipitation and clumping after reconstitution with bacteriostatic water signal either protein aggregation from prior denaturation or particulate contamination

doi:10.1002/lio2.205
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