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Real-time quantitative PCRs (qPCR) were performed by using ABsolute qPCR SYBR Green Mixes (#AB1163A, Thermo Fisher Scientific) according to the manufacturers instructions

Diabetes 63 , 24022414 (2014)

doi: 10.1002/jps.2600590215

Generally, antibodies are categorized into those that target all lactylation modifications, such as Pan-Kla, and those that target specific sites, such as anti-H3K18la
