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Real-time quantitative PCR (RT-qPCR) The total RNA was extracted from leukocytes using the QIAamp RNA Blood Kit (Qiagen, Hilden, Germany), and cDNA was produced with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Waltham, MA) as previously described 18

Cartilage regeneration is a complex process requiring chondrocyte (cartilage cell) proliferation, appropriate extracellular matrix synthesis, and proper tissue organization

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A single 50 mg vial provides 1020 days of research material at these doses

Scientific References Vitamin B12 deficiency in over 16s: diagnosis and management