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doi: 10.1186/s13059-019-1831-z [DOI] [PMC free article] [PubMed] [Google Scholar] 68.Zhao D, Guallar E, Ouyang P, et al

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For fluorescent labelling of UB, Cys-UB and Cys-UB K0 (1 mM) were buffer-exchanged using a PD MidiTrap G-25 column (Cytiva, 28918008) into 25 mM HEPES, 150 mM NaCl and 0.5 mM TCEP and incubated with 5-(Iodoacetamido)fluorescein (5 mM, Sigma-Aldrich, I9271) for 18 h at 4 C

Alternatively, the vascular theory posits optic nerve head atrophy due to insufficient blood supply leading to RGC injury [400]

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Supporting up-and-coming podcasters Welcome to the KILLR Podcast Network Discover how Kail Lowrys podcasting journey defied expectations and led to the creation of the KILLR Podcast Network, where she mentors up-and-coming podcasters and helps them discover their unique niches in the podcasting world