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DNA was extracted from the bead-treated suspension using phenol-chloroform extraction and isopropanol precipitation

Dietary modifications and supportive therapies may also be necessary to manage symptoms and prevent complications

[9] Bloomer et al. Glycine propionyl-L-carnitine modulates lipid peroxidation and nitric oxide in human subjects. International Journal of Vitamin and Nutrition Research. 2009 May;79(3):131-41. doi: 10.1024/0300-9831.79.3.131

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Conclusion The ZSF1 rat model mirrors many alterations observed in human patients, making it a well-suited choice to investigate the connection between the gut microbiome and its metabolites during the manifestation and progression of HFpEF with minimial influencing factors