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Nat Med 25:165175 Lu J, Zuo Y (2017) Clustered structural and functional plasticity of dendritic spines

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An equal volume of 2 EdU working solution was added to the culture medium and incubated for 2 h

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A total of 100 M of the peptides, glutaryl-CoA or succinyl-CoA were added to 100 nM ABHD11-Flag (50 mM TrisHCl pH 7.4, 100 mM NaCl) and 0.2 mM DTNB reagent in a 96-well plate on ice