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Separate-vial workflow Staggered Co-Titration - Two Vials, Two Schedules Run retatrutide alone for 8 weeks, then add cagrilintide at the lowest dose

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Prolonged anesthesia Six week old mice were induced in an anesthesia chamber with 4% isoflurane in oxygen, followed by maintenance anesthesia via facemask with 1.5% isoflurane