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Overexpressed IGFBP-1 in transgenic mice shows that its increase produces hyperinsulinaemia accompanied by glucose intolerance, and that this process is dependent upon IGFBP-1 phosphorylation statewhich is increased in diabetes [259]

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This framework supports reliable physicochemical performance, including solubility behavior and stability in experimental conditions, enabling consistent results in signaling, metabolic, and biochemical assays