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TNF- has been shown, mainly in experimental ED models and chronic inflammatory or metabolic conditions, to activate caspase-dependent apoptotic pathways while inhibiting the PI3K/Akt survival pathway (134)

Tissue repair typically follows a defined temporal sequence after skin or muscle injury, with an inflammatory phase lasting 12 days, a proliferative phase peaking within days, and a remodeling phase extending over months (58)

These findings were further supported by the analysis of the trends with mAUC, which showed no statistically significant difference between groups (p fT3 = 0.147 and p fT4 = 0.132) (Supplementary Figures S3a and S4a)

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