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PloS One 9(10):e109753 Hinz B et al (2007) The myofibroblast: one function, multiple origins

189 Multiple tissue-derived tumor samples show a large number of Arg1-positive myeloid cells in the tumor microenvironment

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Furthermore, mechanical ventilation is a risk factor for cerebral inflammation and brain injury due both to the pulmonary inflammatory cascade, which migrates systemically to the brain, and to hemodynamic instability for the reduction in cardiac output and high pulmonary resistance caused by the distension of alveoli and compression of pulmonary capillaries 132

The desalted sample was evaporated to dryness in a speedvac and then resuspended in 10 L Buffer A and analyzed by mass-spectrometry using the following LC-MS gradient: 5% buffer B in buffer A from 0-15 min, 515% buffer B from 1517.5 min, 1535% buffer B from 17.592.5 min, 3595% buffer B from 92.595 min, 95% buffer B from 95105 min, 955% buffer B from 105107 min, and 5% buffer B from 107125 min