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Protein extraction and western blot analysis The muscle (soleus) tissue samples (35 mg) were homogenized in 700 L EzRIPA lysis buffer (ATTO Biotechnology, Sungnam, Korea) using a mortar and TissueRuptor (QIAGEN, Germany)

Funding This work was funded by the National Natural Science Foundation of China (No.82160072) and the Science and Technology Support Project of Enshi Prefecture Science and Technology Bureau (No.E20180003)

This study examined their co-morbid cerebrovascular effects during abstinence from self-administered Meth after the exposure to chronic unpredictable stress (CUS)

Ascorbic acid was approved by the FDA in 1939